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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Picking Selected Panel Cells Conservatively

Choose cells that can help rule out more than one antibody at a time in order to help decrease supply usage and tech prep time.
Example: Ruling out Anti-C, -Fyb, and -M if you have a suspected Anti-Jka
Panel cell 9+00+0++0
Panel cell 10+0++0+0+
Panel cell 11++0+0++0
Panel cell 12++++0++0
Instead of running 3 separate cells to rule out the antibodies, you can choose one that is homozygous positive for M, C, Fyb and negative for Jka. Panel cell 9 works in this case.
If the only antibody that is present is Anti-Jka, then your test results should be negative. If the results are positive then further rule outs will be needed to determine what is present.