Picking Selected Panel Cells Conservatively

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Picking Selected Panel Cells Conservatively

Choose cells that can help rule out more than one antibody at a time in order to help decrease supply usage and prep time.
Example: You suspect a patient has developed anti-Jka and you'd like to be as efficient as possible in both time and utilization of resources. You have ruled out all other antigens except for C, Fyb, and M. To optimize your process, selecting cell 9 which contains homozygous expressions of each emerges as the most suitable choice.
Rh-HrDuffyKiddMNSs
Panel CellCcFyaFybJkaJkbMN
9+00+0++0
10+0++0+0+
11++0+0++0
12++++0++0
Instead of running 3 separate cells to rule out the antibodies, you can choose one that is homozygous positive for C, Fyb, and M, and negative for Jka. Panel cell 9 works in this case.
If the only antibody that is present is anti-Jka, then your test results should be negative. If the results are positive then further rule-outs will be needed to determine what is present.