Initial Observations of Antibody Panel

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Initial Observations of Antibody Panel

In this slide, we'll explore examples that demonstrate how our prior discussion from the last page can aid in characterizing the reactivity of a suspected antibody.
  • Look at the phase in which reactions are occurring. Reactions at immediate spin (IS) usually are not clinically significant. Reactions at AHG are clinically significant.
  • Look at the strengths of reactions. Varying strengths of reactions could indicate dosage. In the example below, the heterozygous (Jka Jkb) cells react weaker with a 2+ reaction. The homozygous cells (Jkb Jkb) react stronger with a 3+ reaction.
  • Varying strengths may also mean more than one antibody is present.
Rh-HrKellDuffyKiddLewisMNSsP1Lutheran
Donor Cell NumberDCcEeCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsP1LuaLubISAHGCC
1++00+00+0+0++0+++0+++++0+02+NT
2++00++++0+0+++0+0+0++++0+03+NT
3+0++000+0+0+0+0+0++00++0+03+NT
4+0+0+00+0+0+00+00++++0+0+002+
50++0+00+0+0++++++00+0+00+02+NT
600+++0++0+0+0+++0+++++00+02+NT
700+0+00+0+0++00++0+00++0+03+NT
800+0+00+0+0+++0+0+++++00+03+NT
900+0+00+0+0++++00+0++0+0+002+
10+0+0+0++0+0+0+0+0+0++0+0+03+NT
AC002+
IS = immediate spin; AHG = antihuman globulin; CC = check cells; NT = not tested; AC = autocontrol