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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Initial Observations of Antibody Panel

  • Look at the phase in which reactions are occurring. Reactions at immediate spin (IS) usually are not clinically significant. Reactions at AHG are clinically significant.
  • Look at the strengths of reactions. Varying strengths of reactions could indicate dosage. In the example below, the heterozygous (Jka Jkb) cells react weaker with a 2+ reaction. The homozygous cells (Jkb Jkb) react stronger with a 3+ reaction.
  • Varying strengths may also mean more than one antibody is present.