Example of Naturally Occurring Antibody - LabCE.com, Laboratory Continuing Education

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Example of Naturally Occurring Antibody

In this example, anti-M can be identified as the suspected antibody by examining the patterns of reactivity. Reactions, as noted in deep blue, are only occurring at immediate spin, so this would likely not be considered a clinically significant antibody. Clinically significant antibodies are usually IgG and react at 37°C and at the AHG phase.

Rh-Hr

Kell

Duffy

Kidd

Lewis

MNSs

Lutheran

Vial

Donor

C^w

Kp^a

Kp^b

Js^a

Js^b

Fy^a

Fy^b

Jk^a

Jk^b

Le^a

Le^b

Lu^a

Lu^b

AHG

R1R1

R2R2

	Rh-Hr						Kell						Duffy		Kidd		Lewis		MNSs				P	Lutheran
Donor Cell Number	D	C	c	E	e	C^w	K	k	Kp^a	Kp^b	Js^a	Js^b	Fy^a	Fy^b	Jk^a	Jk^b	Le^a	Le^b	M	N	S	s	P₁	Lu^a	Lu^b	IS	AHG	CC
1	+	+	0	0	+	0	0	+	0	+	0	+	+	0	+	+	+	0	+	+	+	+	+	0	+	1+	0	2+
2	+	+	0	0	+	+	+	+	0	+	0	+	+	+	0	+	0	+	0	+	+	+	+	0	+	0	0	2+
3	+	0	+	+	0	0	0	+	0	+	0	+	0	+	0	+	0	+	+	0	0	+	+	0	+	1+	0	2+
4	+	0	+	0	+	0	0	+	0	+	0	+	0	0	+	0	0	+	+	+	+	0	+	0	+	1+	0	2+
5	0	+	+	0	+	0	0	+	0	+	0	+	+	+	+	+	+	0	0	+	0	+	0	0	+	0	0	2+
6	0	0	+	+	+	0	+	+	0	+	0	+	0	+	+	+	0	+	+	+	+	+	0	0	+	1+	0	2+
7	0	0	+	0	+	0	0	+	0	+	0	+	+	0	0	+	+	0	+	0	0	+	+	0	+	1+	0	2+
8	0	0	+	0	+	0	0	+	0	+	0	+	+	+	0	+	0	+	+	+	+	+	0	0	+	1+	0	2+
9	0	0	+	0	+	0	0	+	0	+	0	+	+	+	+	0	0	+	0	+	+	0	+	0	+	0	0	2+
10	+	0	+	0	+	0	+	+	0	+	0	+	0	+	0	+	0	+	0	+	+	0	+	0	+	0	0	2+
AC																										0	0	2+

IS = immediate spin; AHG = antihuman globulin; CC = check cells; NT = not tested; AC = autocontrol