Example of Naturally Occurring Antibody

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The page below is a sample from the LabCE course Antibody Detection and Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Example of Naturally Occurring Antibody

In this example, anti-M can be identified as the suspected antibody by examining the patterns of reactivity. Reactions, as noted in deep blue, are only occurring at immediate spin, so this would likely not be considered a clinically significant antibody. Clinically significant antibodies are usually IgG and react at 37°C and at the AHG phase.
Rh-HrKellDuffyKiddLewisMNSsLutheran
VialDonorDCcEefVCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsLuaLubISAHGCC
IR1R1++00+000++0+0++++00++00+0+2+02+
IIR2R2+0++00000+0+0++0+++0++++0+2+02+
Rh-HrKellDuffyKiddLewisMNSsPLutheran
Donor Cell NumberDCcEeCwKkKpaKpbJsaJsbFyaFybJkaJkbLeaLebMNSsP1LuaLubISAHGCC
1++00+00+0+0++0+++0+++++0+1+02+
2++00++++0+0+++0+0+0++++0+002+
3+0++000+0+0+0+0+0++00++0+1+02+
4+0+0+00+0+0+00+00++++0+0+1+02+
50++0+00+0+0++++++00+0+00+002+
600+++0++0+0+0+++0+++++00+1+02+
700+0+00+0+0++00++0+00++0+1+02+
800+0+00+0+0+++0+0+++++00+1+02+
900+0+00+0+0++++00+0++0+0+002+
10+0+0+0++0+0+0+0+0+0++0+0+002+
AC 002+
IS = immediate spin; AHG = antihuman globulin; CC = check cells; NT = not tested; AC = autocontrol