Mini-Panel Antibody Exclusion

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The page below is a sample from the LabCE course Rh-Negative Mother with Anti-D at Delivery: A Case Study. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Rh-Negative Mother with Anti-D at Delivery: A Case Study (online CE course)
Mini-Panel Antibody Exclusion

Below are the results of a mini-panel of red cells specifically chosen to exclude other clinically significant antibodies in the presence of anti-D.
Besides an autocontrol, a positive control (Ror) was included to confirm that the mother's plasma containing the probable anti-D was reactive at the time of testing.
Recall that the results of the initial antibody screen showed that the possible (unexcluded) antibodies were anti-
D, C, E, K, Fyb, Jka, N, and s (with anti-N less likely as a cause of HDFN).
Antibodies excluded by screen cell #3 included anti-c, e, k, Fya , Jkb, M, S, P1, Lea, and Leb.
Before proceeding to the next page, assess whether the unexcluded antibodies from the initial antibody screen have been excluded by the mini-panel below using the guidelines in the antibody exclusion protocol shown to the right.
CellDCcEeKkFyaFybJkaJkbLeaLebMNSsLuaLubCellGel IAT
100+0++++0+0+00++0+010
200+0+0+0++0++0+0+0020
30+00+0+++0++++0+00030
40++0+++00++00+0++0+40
500++00+0+0++0++0+0050
600+++0+++++0+++++0+60
7+0+++++++++0++0++0+72+
Auto
Auto0
All panel cells are negative for low-frequency antigens and positive for high-frequency antigens unless noted otherwise. All cells are also negative for Cw, Kpa, Jsa, and Lua.