Initial Antibody Exclusions

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The page below is a sample from the LabCE course Rh-Negative Female with Anti-D at Delivery: A Case Study. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Rh-Negative Female with Anti-D at Delivery: A Case Study (online CE course)
Initial Antibody Exclusions

Using the screen cell antigram below, we can see that the antibodies corresponding to the blue-highlighted antigens have been eliminated.

CellRh-HrKellDuffyKiddLewisLutheranPMNSsXg




Rh DCcEeCwKkKpaJsaFyaFybJkaJkbLeaLebLuaLubPMNSsXgaIS37°AHGCC
1R1R1++00+00+00+++++00++0+0+0001+NT
2R2R2+0++00++000++00+0+++++++002+NT
3rr00+0+00+00+00+0+0+0+0+00000
Auto


000
Screen Cell #3 is from a donor who is likely homozygous for c, e, k, Fya , Jkb, Leb, M, S, and Lub AND therefore excludes the corresponding antibodies. Anti-P1 and anti-Lea can be excluded by a single negative with an antigen positive red cell. In the antigram image on the right, all excluded cells are colored blue.
Therefore, possible (not excluded) antibodies include anti-C, D, E, K, Fyb, Jka, N, s, Lea.
Of these anti-Lea is not a cause of HDFN. Lea is poorly developed on fetal RBCs and the antibodies are not considered to be significant when identified in maternal serum.