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The page below is a sample from the LabCE course Theoretical and Practical Aspects of Routine H&E Staining. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Progressive or Regressive Hematoxylin Staining

Most hematoxylin stains can be used as either progressive or regressive stains. Listed below are some common hematoxylin stains and their typical protocol:

Progressive:

  • Mayer's
  • Gill's

Regressive:

  • Harris's
  • Delafield's
  • Ehrilich's