Image 1 represents the cell scatter. The gated population is what determines the fluorescent data represented in the second image.
Image 1. Note: FSC-A and SSC-A are abbreviations for forward and side scatter, respectively.
Image 2 contains the fluorescence data from CD45 staining. Note that virtually all cells in the gated population have CD45 surface antigens and are all leukocytes (white blood cells).
Image 2.
