The following basic associations can be made between certain surface markers and cell types. Please note that only the most basic associated cell types are addressed as this is an introductory flow cytometry course.
A PDF file of this table is also available on this page and can be printed to use as a reference for case studies presented later in this course.
|CD2||Pan (across all) T cells; natural killer cells|
|CD3||Pan T cells|
|CD5||Pan T cells and B cell abnormalities (e.g., B-chronic lymphocytic leukemia (B CLL) and mantle cells)|
|CD7||Pan T cells (earliest marker)|
|CD10 also known as common acute lymphocytic leukemia antigen (CALLA)||Early T and B cells, mature follicular cells|
|CD19||Pan B cells (earliest marker)|
|HLA-DR||B cells (also present on activated T cells)|
|CD23||B cells (present in B CLL and not present in mantle cells)|
|Kappa or Lambda light chain immunoglobulin (not antigen)||B cells --A mature B cell should express either one or the other and, across a B-cell population, there should be a good mix of both Kappa and Lambda. The normal kappa: lambda ratio is approximately 2:1. In a clonal (cancerous) population, one cell line will proliferate and that line will exhibit one of these light chains. This will indicate clonality.|
|CD45 also known as the leukocyte common antigen||Pan white blood cells (May vary in staining intensity between mature and immature white blood cells).|