Staining: Monoclonal Antibody Binding

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The page below is a sample from the LabCE course Introduction to Flow Cytometry: Blood Cell Identification. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Introduction to Flow Cytometry: Blood Cell Identification (online CE course)
Staining: Monoclonal Antibody Binding

To illustrate the reactions which occur inside of the tube containing patient cells and MoAb reagents, please view the video shown. Target cells are bound with MoAbs tagged with fluorochromes against the CD markers of interest. These fluorochromes will provide a means for identification and quantitation by the flow cytometry instrumentation during the analysis phase.

*Used with permission by Dr. Georg Slavka,