Mature T cells can be divided into three major types:
- Helper T cells
- Cytotoxic T cells
- Regulatory T cells
The orange-labeled MoAbs are specific for the CD3 antigen on T cells, and the green-labeled MoAbs are specific for the CD4 antigen on T cells. When a true T-helper cell is analyzed in the flow cytometer, it emits both colors after it passes through the laser, as illustrated in the image on the right.