Clostridium septicum Micromethod Panel Test

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The page below is a sample from the LabCE course Case Studies in Clinical Microbiology. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Clostridium septicum Micromethod Panel Test

  • The definitive identification of C. septicum can be made using a profile of biochemical reactions, as is contained in the micromethod strip seen in the image. (This qualitative micromethod panel test employs conventional and chromogenic substrates for the identification of medically important anaerobes.) The upper set of tubules are reactions before addition of reagents; the bottom set of reactions after reagents are added.
  • The upper set of letter codes is used to read the reactions before addition of reagents; the lower set of labels indicate the tests to read following addition of reagents.
  • Of all the reactions included, only ONPG and NAG in the upper set are positive.
  • The biotype number derived from this profile of reactions, 014000 codes for Clostridium septicum, thus confirming the identification.