Oxidase
A positive spot oxidase reaction is helpful in ruling out the oxidase-negative strains of Capnocytophaga ochracea and Acinetobacter baumannii, which in Gram stain may simulate Neisseria gonorrhoeae. Both Moraxella catarrhalis and Kingella kingae are oxidase positive; therefore, other tests must be used to differentiate these two species.
| Neisseria "Look-Alike" Organisms | Oxidase Result |
| A. baumanii | Negative |
| C. ochracea | Negative |
| K. kingae | Positive |
| M. catarrhalis | Positive |
Superoxol
Superoxol is an additional spot test that may be helpful in the presumptive identification of N. gonorrhoeae.
Superoxol is 30% hydrogen peroxide, in contrast to the 3% solution that is used in the catalase test. Other Neisseria species and M. catarrhalis are either negative for this test or give a weak, delayed reaction.
