The acquisition and implementation of MALDI-TOF MS in the clinical microbiology laboratory requires considerable investigation of resources, workflow, and time. The various preexamination, examination, and postexamination factors discussed in this course provide a concise framework for bringing MALDI-TOF MS into the routine laboratory. To these authors, the many advantages of MALDI-TOF MS, which include a rapid, cost-effective, and accurate means of identification for a wide range of microorganisms, warrant the consideration of this new revolutionary technology.