We can use the forward type with the reverse type to interpret the ABO group. The expected reactions are listed in Table 7.
Table 7. Interpretation of ABO Group.| Red Cells Tested with Known Antisera | Serum Tested with Known Red Cells | Interpretation of ABO Group |
| Anti-A | Anti-B | Anti-A,B | A1 Cells | B Cells |
3–4+ | 0 | 3–4+ | 0 | 3–4+ | A |
0 | 3–4+ | 3–4+ | 3–4+ | 0 | B |
0 | 0 | 0 | 3–4+ | 3–4+ | O |
3–4+ | 3–4+ | 3–4+ | 0 | 0 | AB |
+ = agglutination (graded 1+ to 4+); 0 = no agglutination or hemolysis
