Interpretation of ABO Group

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The page below is a sample from the LabCE course Introduction to the ABO Blood Group System. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Interpretation of ABO Group

We can use the forward type together with the reverse type to interpret the ABO group. The expected reaction are as follows:
Red Cells Tested With Known Antisera
Serum Tested With Known Red Cells
Interpretation of ABO Group
Anti-A
Anti-B
Anti-A,B
A1 Cells
B Cells
3-4+
0
3-4+
0
3-4+
A
0
3-4+
3-4+
3-4+
0
B
0
0
0
3-4+
3-4+
O
3-4+
3-4+
3-4+
0
0
AB
+ = agglutination (graded 1+ to 4+)
0 = no agglutination or hemolysis