The best course of action to learn if CRISPR/Cas9 or CRISPR/Cpf1 has accurately edited the gene or genes of interest is DNA sequencing. High throughput with high accuracy, capable of whole genome sequencing as well as focused regional sequencing, next-generation sequencing (NGS) is the go-to technology.38,39
Of note, CRISPR-mediated gene editing for cancer treatment as a stand-alone exploration is a burgeoning field of studies in basic research as well as in translational research laboratories. More detailed information is expected in the coming decade.
38. Bock, C., Datlinger, P., Chardon, F., Coelho, M. A., Dong, M. B., Lawson, K. A., Lu, T., Maroc, L., Norman, T. M., Song, B., Stanley, G., Chen, S., Garnett, M., Li, W., Moffat, J., Qi, L. S., Shapiro, R. S., Shendure, J., Weissman, J. S., & Zhuang, X. (2022). High-content CRISPR screening. Nature Reviews Methods Primers, 2(1). https://doi.org/10.1038/s43586-021-00093-439. DeWitt, J., PhD. (n.d.). CRISPR off-target detection with NGS. Integrated DNA Technologies. https://www.idtdna.com/pages/technology/crispr/crispr-genome-editing/crispr-detection/next-generation-sequencing
