Microscopic Morphologic Features of the Yeast Forms of Various Dimorphic Fungi

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The page below is a sample from the LabCE course Mycology: Yeasts and Dimorphic Pathogens. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Microscopic Morphologic Features of the Yeast Forms of Various Dimorphic Fungi

The yeast form of Blastomyces dermatitidis is a large, 10-15 µm in diameter, thick-walled yeast cell that characteristically produces a single bud attached by a broad base (Image E).
Coccidioides immitis does not produce a yeast form in laboratory culture; rather, is identified in stained tissue sections by the production of varying-sized spherules, ranging from 10 µm to over 100 µm in diameter (Image F). The larger, more mature spherules contain spherical endospores ranging from 2-4 µm in diameter.
Paracoccidioides brasiliensis also produces large yeast cells approximately the same size as those of Blastomyces dermatitidis, except multiple buds are attached by narrow necks, giving the appearance of a "mariner's wheel" (Image G).
The yeast forms of Sporothrix schenckii are elongated cells that have been called "cigar bodies" (Image H).