As a gene-editing tool, the CRISPR effect centers around the type of DNA repair mechanism. Here's a closer look at this idea:13
- Directed by gRNA (the general term for either single RNA or dual tracrRNA-crRNA), CRISPR/Cas9 carries out DNA breakage in both strands, also referred to as double-stranded breaks.
- This genomic damage immediately alerts DNA repair mechanisms to take action.
13. Xue, C., & Greene, E. C. (2021). DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing. Trends in genetics: TIG, 37(7), 639–656. https://doi.org/10.1016/j.tig.2021.02.008
