Noticing the panreactive but variable reactivity, the reference laboratory decided to perform a full serological phenotype on the patient and run several phenotypically similar cells to determine if the reactivity was caused by a combination of common red cell antibodies, or by an antibody to a high-prevalence antigen.
Patient's ABO/Rh and serological phenotype: O-; C- E- c+ e+; K- k+; Fy(a-b+); (Jka+b+); M+ N+; S- s+
| Rh-Hr | Kell | Duffy | Kidd | Lewis | MNSs | P1 | Lutheran | Xg | |
Cell Number | D | C | c | E | e | V | Cw | K | k | Kpa | Kpb | Jsa | Jsb | Fya | Fyb | Jka | Jkb | Lea | Leb | M | N | S | s | P1 | Lua | Lub | Xga | AHG (PEG) |
1 | 0 | 0 | + | 0 | + | 0 | 0 | 0 | + | 0 | + | 0 | + | 0 | + | + | + | + | 0 | + | + | 0 | + | + | 0 | + | + | 1+ |
2 | 0 | 0 | + | 0 | + | 0 | 0 | 0 | + | 0 | + | + | + | 0 | 0 | + | 0 | 0 | 0 | 0 | + | 0 | + | + | 0 | + | + | w+ |
3 | 0 | 0 | + | 0 | + | 0 | 0 | 0 | + | 0 | + | 0 | + | 0 | + | 0 | + | 0 | + | + | 0 | 0 | + | + | 0 | + | 0 | 1+ |
Note that the highlighted cells are the antigens which match the patient.