Case Study 3: Chemical Treatment - LabCE.com, Laboratory Continuing Education

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The page below is a sample from the LabCE course A Comprehensive Overview of "HTLA" and "HTLA-Like" Antibodies. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Case Study 3: Chemical Treatment

The next step taken was to treat the cells from the initial panel with 0.2M DTT:

	Rh-Hr							Kell						Duffy		Kidd		Lewis		MNSs				P₁	Lutheran		Xg
Cell Number	D	C	c	E	e	V	C^w	K	k	Kp^a	Kp^b	Js^a	Js^b	Fy^a	Fy^b	Jk^a	Jk^b	Le^a	Le^b	M	N	S	s	P₁	Lu^a	Lu^b	Xg^a	AHG (DTT)
1	+	+	0	0	+	0	0	0	+	0	+	0	+	+	0	+	+	+	0	+	+	+	+	+	0	+	0	0√
2	+	+	0	0	+	0	+	+	+	0	+	0	+	+	+	0	+	0	+	0	+	+	+	+	0	+	0	0√
3	+	0	+	+	0	0	0	0	+	0	+	0	+	0	+	0	+	0	+	+	0	0	+	+	0	+	0	0√
4	+	0	+	0	+	+	0	0	+	0	+	0	+	0	0	+	0	0	+	+	+	+	0	+	0	+	0	0√
5	0	+	+	0	+	0	0	0	+	0	+	0	+	+	+	+	+	+	0	0	+	0	+	0	0	+	0	0√
6	0	+	0	+	+	0	0	+	+	0	+	0	+	0	+	+	0	0	+	+	+	+	+	0	0	+	0	0√
7	0	0	+	0	+	0	0	0	+	0	+	0	+	+	0	+	0	+	0	+	0	0	+	+	0	+	+	0√
8	0	0	+	0	+	0	0	0	+	0	+	0	+	+	+	0	+	0	+	+	+	+	+	0	0	+	0	0√
9	0	0	+	0	+	+	0	0	+	0	+	0	+	+	+	+	0	0	+	0	+	+	0	+	0	+	0	0√
10	+	0	+	0	+	0	0	+	+	0	+	0	+	0	+	0	+	0	+	0	+	+	0	+	0	+	+	0√
AC																												0√

The results of this panel show that the antigen is destroyed by 0.2M DTT treatment, further narrowing down the possibilities to the identity of the antibody.