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Expected Pathogens From Common Body Sites/Specimens
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Reading Gram-stained Smears From Cultures
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Expected Pathogens From Common Body Sites/Specimens
Body Site/Specimen
Organism(s) Commonly Expected
Comments
Blood
Staphylococcus
species: Gram-positive cocci (GPC) in clusters and tetrads
Streptococcus
or
Enterococcus
species: GPC in pairs and long chains;
S. pneumoniae
as GPC in lancet-shaped pairs
Haemophilus
species: Pale pink, gram-negative coccobacilli (GNCB)
Pseudomonas, e
nteric organisms (eg
E.coli
), and
Bacteroides
species: Gram-negative rods (GNRs)
Clostridium
or
Listeria
species: Gram-positive rods (GPRs)
Yeast
Generally, only one morphotype is expected in sterile body sites.
GPC are the most commonly isolated organisms in blood, including coagulase (COAG)-negative staph, S. aureus, E
nterococcus
species, viridans streptococci,
S. pneumoniae
, and beta-hemolytic streptococci.
Other commonly isolated pathogens include
E. coli
,
Candida albicans
,
P. aeruginosa
,
Klebsiella pneumoniae
,
Enterobacter cloacae
,
Proteus
species, and anaerobic bacteria.
Yeast may be present in the blood cultures of immunocompromised patients
Cerebrospinal fluid (CSF)
Neisseria
species: Gram-negative diplococci (GNDC)
Haemophilus
species: Pale pink gram-negative coccobacilli (GNCB)
Streptococcus
species: GPC in pairs (diplococci) and long chains
Staphylococcus
species: GPC in clusters and tetrads
Listeria
: Gram-positive rods (GPR)
Generally, only one morphotype is expected in sterile body sites.
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