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Gram-negative Organisms Recovered From Culture
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Reading Gram-stained Smears From Cultures
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Gram-negative Organisms Recovered From Culture
Oxygen Requirement/Gram Stain Characteristics
Common Genus
Characteristic Gram Stain Morphology/Comments
Aerobic, gram-negative cocci (GNC)
Neisseria
Moraxella catarrhalis
Gram-negative diplococci (pairs) with adjacent sides flattened (coffee bean shaped)
Aerobic, gram-negative rods (GNRs) and coccobacilli
Pseudomonas
Haemophilus
Pseudomonas
: Straight, slender, gram-negative rods (GNRs).
H. influenzae
: Small , pale staining, coccobacilli or short GNRs.
Anaerobic, GNRs
Bacteroides
Fusobacterium
B. fragilis
: Pale-staining, pleomorphic rods with rounded ends.
F. nucleatum
subspecies
nucleatum
: Pleomorphic GNRs with round to tapered ends.
Facultative anaerobic GNRs
Enterics (eg,
Escherichia, Klebsiella, Proteus, Enterobacter, Salmonella, Shigella
)
Typically, the enterics are plump GNRs.
There is not a unique morphology that can be used to distinguish between the enteric organisms.
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