Pair Cell Arrangement

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The page below is a sample from the LabCE course Reading Gram-stained Smears From Cultures. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Pair Cell Arrangement

This field shows examples of gram-positive bacteria in pairs, often referred to as diplococci. These are lancet-shaped "diplococci" which could represent Streptococcus pneumoniae. Although the Gram stain is characteristic of S. pneumoniae, the determination of bacterial species always requires confirmatory testing. This particular image is a direct Gram stain prepared from a sputum specimen and does not represent organisms recovered from culture. The large gram-negative, red to pink staining rounded structures in the background are polymorphonuclear (PMN) leukocytes.