Properly Decolorized Smears

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The page below is a sample from the LabCE course Reading and Reporting Gram-stained Direct Smears. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Reading and Reporting Gram-stained Direct Smears (online CE course)
Properly Decolorized Smears

In a properly decolorized smear, background material and host cells (eg, white blood cells and epithelial cells) stain pink or red, as illustrated in this image. The field that is shown is a good example of one appropriate for microscopic examination. Note that the stained material is only one cell thick; therefore, cells can be counted and identified easily.