Real-Time PCR (Online CE Course)

(based on 292 customer ratings)

Coauthors: Heather MacDonald, M(ASCP), MB(ASCP) and Ryanne Wolfe, MS, MBA
Reviewer: David J. Moffa, PhD, BCLD

How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 136 CE courses, most popular
$95Add to cart
Pick Your Courses
Up to 8 CE hours
$50Add to cart
Individual course$20Add to cart

This course provides a brief overview of DNA and RNA structure and the principles of polymerase chain reaction. It then proceeds to discuss the principles and procedures for real-time PCR, highlighting the advantages over traditional PCR methods and the applications in the clinical setting.

See all available courses

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Approved through 6/30/2022
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Molecular Pathology): 1 hour(s)
Approved through 9/1/2022

Objectives

  • Compare and contrast the structure and functional regulation of DNA and RNA.
  • Discuss the basic principles of the polymerase chain reaction (PCR) process.
  • Define the detection techniques for the measurement of nucleic acids by real-time PCR.
  • Understand the evolution of traditional PCR to real-time PCR, and the advantages and limitations of both methods.
  • Discuss the applications of real-time PCR.

Customer Ratings

(based on 292 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • Historical landmarks in Molecular Diagnostics
      • Historical Landmarks in Molecular Diagnostics
  • Composition and Formation of DNA and RNA
      • Molecular Composition of DNA
      • Molecular Composition of RNA
      • Formation of DNA and RNA Strands
      • Which two of the following characteristics are common to both RNA and DNA?
      • The DNA strand ending with a phosphate group is the 5' end.
  • Extraction and Isolation of Genetic Material
      • Nucleic Acid Isolation
      • Automated Vs. Manual Extraction
      • There are several methods for extracting nucleic acids (DNA and/or RNA}) from samples. The most common protocols are manual or automated which essenti...
  • Introduction to Polymerase Chain Reaction (PCR)
  • Real-time PCR
    • Primers
      • Primers
    • Polymerase
    • Enzyme Cofactors, dNTPs, and Salts/Buffers
      • Enzyme Cofactors, dNTPs, and Salts/Buffers
      • Reverse Transcriptase PCR
    • Thermocyclers and Detection Methods
      • Thermocyclers
      • Detection Methods
      • Phases of Real-Time PCR
      • During which phase in real-time PCR can the fluorescent signal start to be recorded?
  • Applications of Real-Time PCR
      • Laboratory Applications
      • Multiplexing
      • Microbe Detection
      • SNP Detection
      • Viral Load
      • One of the best ways to evaluate single nucleotide polymorphism (SNP) is by melting curve analysis
      • Which is not an application of real-time PCR?
  • Conclusion
      • Conclusion
  • References
      • References

Additional Information

Level of instruction: Intermediate

Intended audience: Medical laboratory scientists, medical technologists, and technicians, This course is also appropriate for clinical laboratory science students and pathology residents. 
 
Author information: Heather MacDonald, M(ASCP), MB(ASCP) has over 10 years of clinical laboratory experience and oversees a Molecular Diagnostics Laboratory. She is currently the Advanced Diagnostics Manager for Children's Healthcare of Atlanta in Georgia. Along with performing routine diagnostic assays, implementing laboratory-developed qualitative and quantitative molecular assays (both singleplex and multiplex) is her primary focus. Heather has published numerous articles and presents her research at national and international meetings. She has also worked with numerous corporations to bring commercial assays to market.
 
Coauthor information: Ryanne Wolfe holds an MS in biotechnology and an MBA from the University of Maryland. Ryanne has worked in the health care field for eight years, most recently in the laboratory at St. Christopher’s Hospital for Children in Philadelphia, Pennsylvania.
 
Reviewer information: David Moffa, PhD, has over 30 years of experience in the health care industry as an executive manager, clinical laboratory director, and medical laboratory scientist. He is currently a technical consultant for Kentmere Healthcare, Wilmington, DE, and until his retirement, was the Regional Director for LabCorp, Inc. He holds a PhD in medical biochemistry from the School of Medicine, West Virginia University.
 
Course information: This course provides a brief overview of DNA and RNA structure and the principles of polymerase chain reaction. It then proceeds to discuss the principles and procedures for real-time PCR, highlighting the advantages over traditional PCR methods and the applications in the clinical setting.

How to Subscribe
MLS & MLT Comprehensive CE Package
Includes 136 CE courses, most popular
$95Add to cart
Pick Your Courses
Up to 8 CE hours
$50Add to cart
Individual course$20Add to cart
110220 course cover


DNA Structure


RNA-DNA-copying.JPG


FRET PROBE