Course Outline
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- Importance of Fluorescence In Situ Hybridization (FISH) Testing in Cancer Care
- Detecting Genetic Aberrations with Fluorescence In Situ Hybridization (FISH)
- Drug Therapy Based on Genetic Aberrations
- DNA Content and Ploidy
- Genetic Aberrations
- Traditional Cytogenetics
- Metaphase Fluorescence In Situ Hybridization (FISH)
- Interphase Fluorescence In Situ Hybridization (FISH) in Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue
- What type of fluorescence in situ hybridization (FISH) is performed on formalin-fixed, paraffin-embedded (FFPE) tissue?
- Theoretical Knowledge Required for Fluorescence In Situ Hybridization (FISH) Testing
- Equipment used in FISH technology
- Equipment Required to Prepare and Store Reagents
- Equipment Required for Preparation of Tissue
- Equipment Required for Manual Performance of Fluorescence In Situ Hybridization (FISH) Assay
- Equipment Required for Analysis of Fluorescence In Situ Hybridization (FISH) Slides
- Photographing Fluorescence In Situ Hybridization (FISH) Slides
- All of the following are REQUIRED in order to perform a fluorescence in situ hybridization (FISH) assay EXCEPT:
- FISH methodology
- Enumeration of FISH signals
- Assessing Slide Quality
- Control Slide
- Patient Slide
- HER2/neu Examples
- Reporting Fluorescence In Situ Hybridization (FISH)
- International Standing Committee on Cytogenetic Nomenclature (ISCN) Reporting
- A final interpretation of FISH results is required in the report.
- The following image is of a break apart probe for ALK translocation. Is the image depicting a normal or an abnormal sample?
- References
Additional Information
Level of instruction: Beginning to intermediate
Intended audience: Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.
Author information: Kathryn R. Perkinson, BS, HTL(ASCP) has been the manager of the Molecular Pathology Department of Duke University Health System in Durham, North Carolina since 1998. She holds a Bachelor of Science degree in biochemistry from North Carolina State University. Kathryn has over 25 years of experience in molecular biology in both the research and clinical healthcare fields at Duke University. The Molecular Pathology Department, under her leadership, has tripled in size over the past 15 years.
Reviewer information: Carla J. Shoffeitt, MSM, HT(ASCP) is the Manager of the Pathology Department of Emory St. Joseph’s Hospital of Emory Healthcare in Atlanta, Georgia. She holds a Master of Science Degree in Healthcare Management as well as a Bachelor of Science degree in Biology from Troy University, Troy, Alabama. She is certified as a Histotechnician and has 25 years of experience in the field of Histology.
Course description: This course will explore the theoretical concepts used in fluorescence in situ hybridization (FISH) testing. Commonly used FISH methodologies, necessary equipment, and the enumeration of FISH signals will also be discussed.
