Course Outline
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- Basics of the Immune System
- History of the MHC in Humans and Other Animals
- Discovery of the Major Histocompatibility Complex (MHC) and Human Leukocyte Antigens (HLA)
- HLA 101 Terminology
- Immune System Overview
- Overview of the Immune System
- Overview of Innate Immune System
- True or False: The innate immune response is characterized by antibody production, cell-mediated apoptosis, and memory cell formation towards a specif...
- Overview of Adaptive Immune System
- Review of Cells in Adaptive Immune System: Pathways & Activation
- Match the following cells of the adaptive immune response with their function.
- HLA Nomenclature
- Nomenclature of HLA Glycoproteins
- Nomenclature of HLA Typings: Example
- True or False: The first field of an HLA nomenclature is designated as the allele group or the antigenic level of the protein that is detectable serol...
- Genomic Distribution of the HLA System
- Chromosome Location
- MHC/HLA Location on Human Chromosomes
- Which of the following is not a locus that is found in HLA class I?
- True or False: There are four regions on band 21 of chromosome 6 that give rise to HLA genes.
- Inheritance Patterns
- Various Molecular Structures of the HLA System
- Lymphocytes
- Match the lymphocyte with its cluster of differentiation (CD).
- Class I Structure and Functionality
- Class II Structure and Functionality
- HLA Class II: Structure and Location
- HLA Class II: Functionality
- Class I and II Structure and Function Summary
- True or False: Class I HLAs are expressed on all nucleated cells and present endogenous antigens to lymphocytes. Class II HLAs are expressed on antige...
- Match each of the functionalities described into the appropriate HLA class.
- Methodologies of HLA Analysis
- DNA Extraction and Quantitation
- SSO
- Sequence-Specific Oligonucleotide (SSO)
- SSP
- Types of HLA Crossmatches
- Engraftment Monitoring
- Antibody Screening
- NGS
- HLA's Role in Transplantation, Alloreaction, and Disease Associations
- HLA Functionality and ABO/Rh in Transplantation
- Alloreactivity and Graft Versus Host Disease (GvHD)
- HLA Alleles and Predisposition to Diseases and Drug Reactions
- Match the types of rejection with the definition that meets the criteria for the type of reaction that would occur.
- A deceased donor's kidney becomes available (shown in last row) and four patients are eligible (see image on right). Which patient would be the ideal ...
- References
Additional Information
Level of Instruction: Beginner to Intermediate
Intended Audience: Medical laboratory scientists and technicians and other health care personnel who have an interest in this subject matter. This course is also appropriate for medical laboratory science students and pathology residents.
Author Information: Ethan Kentzel has earned a Master of Science in Biomedicine and Business at Drexel University. He holds a BS degree in Medical Technology from Slippery Rock University and an ASCP MLS certification. Ethan has experience as a medical laboratory scientist working in various labs, including Blood Bank, Immunogenetics/HLA, Microbiology, and briefly as a generalist. While working as a laboratory scientist, he was also an adjunct professor for the Medical Laboratory Science program at St. Christopher's Hospital for Children. He works as a Program Director for the Medical Laboratory Science and Phlebotomy programs at Reading Hospital School of Health Science. He continues to teach at St. Christopher's Hospital for Children and Reading Area Community College.
Reviewer Information:
Andrea G. Gordon, M.Ed, MT(ASCP), SH, has over 25 years of experience as a Program Director for various laboratory science programs. She has approximately 5 years of experience as an administrator in higher education as a VP and Director of Education. Additionally, she has over 15 years of experience as a volunteer for NAACLS.
Marissa Wildung, MLS(ASCP)CM, is a Laboratory Supervisor with nine years of experience as a Medical Laboratory Scientist and a Faculty Instructor for an MLT/Phlebotomy Program in Central Minnesota. Marissa holds a Bachelor's in Medical Laboratory Science from the University of Cincinnati and is pursuing a Master's in Business Administration from St. Cloud State University.
