Course Outline
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- Introduction
- Tissue Staining in the Clinical Histology Laboratory
- The Biological Stain Commission
- True or False: Cellular and intracellular structures of tissue samples cannot be microscopically examined until dyes color them.
- What is a primary function of the Biological Stain Commission?
- Basic Staining Mechanisms
- What Makes a Dye?
- Factors Affecting Dye Binding
- Acids, Bases, pH, and Buffers
- Basophilic and Acidophilic Staining
- Oxidation and Reduction
- True or False: Acidic dyes contain basic groups that have an affinity for acidic tissue elements.
- What is the chemical reaction called that results in the removal of electrons from a molecule?
- Nervous System Overview
- Classifying Nervous Tissue
- Functions of Nervous Tissue
- Histology of the Nervous System: Neurons
- Histology of the Nervous System: Neurofibrillary Tangles
- Histology of the Nervous System: Nissl Substance
- Histology of the Nervous System: Neuroglia
- Histology of the Nervous System: Myelin
- True or False: The central nervous system (CNS) comprises the brain and spinal cord.
- Cell processes arising from the nerve cell body consist of which of the following?
- Special Staining Methods Used to Demonstrate Nervous Tissue
- Using the Microwave for Special Staining Procedures
- References
Additional Information
<div><strong>Level of Instruction</strong>: Intermediate</div>
<div><strong>Intended Audience</strong>: Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and medical laboratory science students, pathology residents, and practicing pathologists. </div>
<div><strong>Author Information</strong>: Tanisha N. Neely, HT(ASCP), is a freelance writer with work history in histology, microbiology, and pharmaceutical research. She attended Indiana University, where she earned a bachelor's degree in arts and humanities from the School of General Studies and a Certificate in Histotechnology from the School of Medicine. She is a member of the National Society for Histotechnology and the Indiana Society for Histotechnology.</div>
<div>The author has no
conflict of interest to disclose.</div>
<div><strong>Reviewer Information</strong>: </div>
<div>Laurie Bjerklie, MA, MLS(ASCP)<sup>CM</sup>, is
a Lead Education Developer. She earned a B.S. in Medical Laboratory Science
from the University of North Dakota and an M.A. in Curriculum and Instruction
from Saint Xavier University. She has over 20 years of experience in higher
education and has held program director and faculty positions in both MLT and
MLS programs.</div>
<div>Carla J. Shoffeitt, MSM, HT(ASCP), is the Manager of the Pathology Department of Emory St. Joseph’s Hospital of Emory Healthcare in Atlanta, Georgia. She holds a Master of Science Degree in Healthcare Management and a Bachelor of Science degree in Biology from Troy University in Troy, Alabama. She is certified as a Histotechnician and has 25 years of experience in the field of Histology. </div>
<div><strong>Course Description</strong>: This is the final course in the series exploring special stains used in the clinical histology laboratory. Basic nervous tissue staining mechanisms and classification of nervous tissue elements will be discussed. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate nervous tissue in samples. </div>
