Course Outline
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- Introduction
- Tissue Staining in the Clinical Histology Laboratory
- The Biological Stain Commission (BSC)
- Cellular and intracellular structure of tissue samples cannot be microscopically examined until they are colored by dyes.
- What is a primary function of the Biological Stain Commission?
- Basic Staining Mechanisms
- What Makes a Dye?
- Factors that Affect Dye Binding
- Acids, Bases, pH, and Buffers
- Basophilic and Acidophilic Staining
- Oxidation and Reduction
- Acidic dyes contain basic groups that have an affinity for acidic tissue elements.
- What is the chemical reaction called that results in the removal of electrons from a molecule?
- Nervous System Overview
- Special Staining Methods Used to Demonstrate Nervous Tissue
- Using the Microwave for Special Staining Procedures
- Microwaves in the Histopathology Laboratory
- Tips for Using the Microwave for Special Staining Procedures
- Safety Precautions for Microwave Usage
- Containers used in a microwave oven should be tightly sealed to prevent evaporation of the solution.
- Microwave ovens heat solutions _____ from the inside out.
- References
Additional Information
Level of instruction: Intermediate
Intended audience: Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and clinical laboratory science students, pathology residents, and practicing pathologists.
Author information: Tanisha N. Neely, HT(ASCP) is a freelance writer with a work history in histology, microbiology, and pharmaceutical research. Ms. Neely attended Indiana University where she earned a bachelors degree in arts & humanities from the School of General Studies and a Certificate in Histotechnology from the School of Medicine. She is a member of the National Society for Histotechnology and the Indiana Society for Histotechnology.
Reviewer information: Diana Harrington, BS, HT(ASCP) is a histotechnologist at The Dermatology Center of Indiana. Since graduating from Indiana University, she has worked in various hospital laboratories as a medical technologist and histotechnologist. Her experience led her to teach IU students as a Clinical Education Supervisor and create the Histotechnology Program as Program Director for Keiser University in Florida.
Reviewer information: Carla J. Shoffeitt, MSM, HT(ASCP) is the Manager of the Pathology Department of Emory St. Joseph’s Hospital of Emory Healthcare in Atlanta, Georgia. She holds a Master of Science Degree in Healthcare Management as well as a Bachelor of Science degree in Biology from Troy University, Troy, Alabama. She is certified as a Histotechnician and has 25 years of experience in the field of Histology.
Course description: This is the final course in the series that will explore special stains used in the clinical histology laboratory. Basic nervous tissue staining mechanisms and classification of nervous tissue elements will be discussed. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate nervous tissue in samples.
