Course Outline
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- Basic Principals of Electrophoresis
- Introduction
- Principle of Electrophoresis
- Amphoteric
- Isoelectric Point (pI)
- Mobility or Rate of Migration
- Rate of Migration
- Which statement is correct for a protein with an isoelectric point (pI) of 7.0?
- The pI of a protein is 9.2. This protein is placed in an electrical field where a buffer sets the pH at 10.0. Select the correct statement regarding t...
- Which one of the following will slow down the migration of solutes in electrophoresis?
- Electrophoresis and Buffers
- Role of Buffers
- Buffers and pH
- Proteins in a buffer with the pH set at 8.6 will become anions and move to the positively charged electrode.
- Specimens for Electrophoresis
- Specimens
- After reviewing the information on specimen samples for electrophoresis, select the one correct statement.
- Electrophoresis and Support Media
- Types of Support Media
- Agarose Gel
- Polyacrylamide Gels
- There are several different types of media that can be used in electrophoresis. Most methods today use a gel (cellulose acetate, agarose, or polyacryl...
- Electrophoresis Instrumentation
- Electrophoresis Equipment
- Automation
- Automated electrophoresis systems only include automated reagent addition, electrophoresis of sample, staining of separated fragments, and detection o...
- Types of Electrophoresis
- Types of Electrophoresis
- Routine Electrophoresis
- High Resolution Electrophoresis (HRE)
- Polyacrylamide Electrophoresis (PAGE)
- Denaturing Polyacrylamide Gels
- Capillary Electrophoresis (CE)
- CE Advantages and Applications
- Isoelectric Focusing (IEF)
- IEF Advantages and Applications
- Immunofixation Electrophoresis
- Pulsed Field Electrophoresis
- Two-Dimensional Electrophoresis
- Two-Dimensional Electrophoresis Advantages and Applications
- Types of Electrophoresis-- Summary
- In isoelectric focusing, the basis of separation of solutes is different than the other types of electrophoresis. Which statement below correctly desc...
- Sodium dodecyl sulfate is added to polyacrylamide gels to denature the proteins in the sample and enhance their separation.
- Visualization and Detection
- Visualization and Detection Methods
- Stains and Dyes
- Densitometry
- Labeled Probes
- Which statements below are correct descriptions of visualization and detection methods used in electrophoresis?
- Technical Considerations and Electrophoresis Troubleshooting
- Technical Considerations and Electrophoresis Troubleshooting Topics
- Sample Application
- Buffers
- Support Media
- Electroendosmosis
- Wick Flow
- Troubleshooting Irregular, Distorted, or Atypical Bands
- Which statements below are associated with electroendosmosis?
- Wick flow is caused by movement of the buffer ions into the medium when there has been a loss of moisture in the medium due to heat generation.
- Resurgence of Electrophoresis
- Resurgence of Electrophoresis
- Electrophoresis and Molecular Diagnostics
- Blotting Techniques
- Uses of CE in Molecular Diagnostics
- References
Additional Information
Level of instruction: Intermediate
Intended Audience: Clinical laboratory technologists and technicians, and other health care personnel who have an interest in this subject matter. This course is also appropriate for clinical laboratory science students and pathology residents.
Author Information: Mary Ellen Koenn, MS, MLS(ASCP) is an associate professor emeritus, West Virginia University School of Medicine, Medical Laboratory Science Program. During her career as a medical technologist and educator, she has been a laboratory supervisor and manager and has held several teaching positions. She is the author of numerous articles for laboratory publications and textbook chapters and is a frequent presenter at laboratory seminars and workshops. Ms. Koenn holds a Master of Science degree in Medical Technology.
Reviewer Information: Leslie Lovett, MS, MT(ASCP) is the Clinical Education Coordinator of the Medical Laboratory Technology Program and a professor at Pierpont Community and Technical College in West Virginia. She holds a MS degree in Medical Technology with classes toward a PhD in Pharmacology and Toxicology.
Reviewer Information: Rory Huschka M.Ed., MLS(ASCP) works on the content development team at MediaLab and LabCE. Mr. Huschka earned is BS degree in Medical Technology from North Dakota State University and later a MA degree in Educational Leadership. He has taught in higher education for over 20 years and continues to work as an adjunct professor for various MLT and MLS programs.
Course Description: This course discusses the many types and applications of electrophoresis in the clinical laboratory. Commonly used terms are defined and procedures are described, including explanations of various electrophoretic patterns. A discussion on newer molecular diagnostic techniques that incorporate electrophoresis conclude this in depth look at electrophoresis.
