Course Outline
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- Case Presentation
- Laboratory Workup
- Identification by Culture Methods
- Case Study, continued: Laboratory Testing
- Case study, continued Upon arrival in the laboratory, one of the swabs is used for culture. Which one of these selective media is an appropriate choic...
- Case Study, continuedAfter four days of incubation at 37°C, small, shiny colonies are observed on the Regan-Lowe agar. A Gram stain of the cultur...
- Nucleic Acid Amplification Testing (NAAT)
- Genomic Insertion Sequences
- Single-Copy Gene Targets
- Positive Predictive Value
- Addressing False-Positive NAAT Results
- True or false: Insertion sequences can move around and insert into different locations on the genome, changing the overall genetic sequence of the bac...
- Direct Fluorescent-Antibody Staining (DFA)
- White Blood Cell (WBC) Count
- Case Study, continued: IgA, IgM and IgG Antibody Testing
- Table 2: Advantages and Disadvantages of Laboratory Tests for Identification of B. pertussis
- Clinical Stages of Pertussis and Optimal Timing for Diagnostic Testing
- Which of these tests may be performed late in the course of B. pertussis infection?
- How does the prevalence of a particular pathogen in a given area correlate to positive predictive values?
- Case study conclusion
- Case Study Conclusion
- Treatment and Dosing for Pertussis
- Prevention
- Which of the following antibiotics is recommended for infants less than one month old for treatment of Bordetella pertussis infection?
- References
Additional Information
Level of instruction: Intermediate
Intended Audience: Medical laboratory scientists, medical laboratory
technicians, laboratory supervisors, and laboratory managers. This course is
also appropriate for MLS and MLT students and pathology residents.
Author information: Leah Ames, MS, MLS(ASCP)CM is a medical technologist in the Molecular Diagnostics department at Alverno Clinical Laboratories. She also serves as adjunct faculty for molecular diagnostics in the CLS program at Northern Illinois University and is an instructor of molecular diagnostics for Franciscan St. Margaret Health School of Medical Technology. Leah holds an M.S. degree in Clinical Laboratory Science from Michigan State University and a B.S. in Molecular and Integrative Physiology from the University of Illinois.
Reviewer information:
Laurie Bjerklie, MA, MLS(ASCP)CM is currently an
Education Developer for MediaLab and LabCE. She earned a B.S. in Medical
Laboratory Science from the University of North Dakota and an M.A. in
Curriculum and Instruction from Saint Xavier University. She has over 15 years
of experience in higher education and has held program director and faculty
positions in both MLT and MLS programs.
Joshua J. Cannon, MS, MLS(ASCP)CMSHCM received his Bachelor
of Science and Master of Science in Medical Laboratory Science from Thomas
Jefferson University in Philadelphia, PA. He holds Medical Laboratory Scientist
and Specialist in Hematology certifications through the ASCP Board of
Certification. He was a professor at Thomas Jefferson University for seven
years before transitioning into his current role as Education Developer at
MediaLab. His areas of expertise and professional passions include clinical
hematology and interprofessional education.