Course Outline
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- General Information Related to Identification of Molds
- Morphologic Features of Yeast
- Preliminary Identification Methods for Yeasts: Wet Mounts and KOH
- Preliminary Identification Methods for Yeasts: India Ink
- Preliminary Identification Methods for Yeasts: Germ Tubes
- Morphologic Features of Yeast Colonies
- Microscopic Morphologic Features of Yeast
- Malassezia
- Which of the organisms listed below has the unique property of producing germ tubes when fetal bovine serum is lightly inoculated with the yeast isola...
- The growth of the yeast-like colonies shown in the upper image was obtained on blood agar from a skin culture only in the area overlaid by virgin oliv...
- Yeast Identification Using Cornmeal Agar
- Interpretation of Growth Patterns on Cornmeal Agar
- Examples of Cornmeal Agar Morphologies
- Match each cornmeal agar morphology listed with the corresponding yeast species.
- Match each of the fungal species with the appropriate category, indicating whether or not it has the capability of producing pseudohyphae on cornmeal ...
- A yeast identification system gave a biotype number for an unknown isolate that did not differentiate between Candida tropicalis and Candida parapsilo...
- Extending the Preliminary Identification to Full Identification
- Evaluating the Need for Full Identification of Yeast
- The Importance of Candida auris
- Recommended Procedure for Identification of Cryptococcus neoformans
- The Importance of Cryptococcus gattii
- Arrange in sequence the steps that should be taken to make an initial identification of Cryptococcus neoformans or Cryptococcus gattii.
- The colonies shown in this photograph were recovered from a blood culture after 48 hours of incubation at 30°C. What is the most likely source of...
- Illustrated in this photomicrograph of a lactophenol blue preparation of urine sediment is a cluster of yeast cells that were presumptively identified...
- Which yeast is easily misidentified, may be difficult to treat, grows at 42 °C and on cycloheximide, and requires MALDI-TOF MS or molecular metho...
- Which yeast can be differentiated from Cryptococcus neoformans only by using MALDI-TOF MS, nucleic acid–based methods, or CGB agar (for melanin-...
- Dimorphic Pathogens
- What are Dimorphic Fungi?
- Microscopic Morphologic Features of the Mold Forms of Various Dimorphic Fungi
- Microscopic Morphologic Features of the Yeast Forms of Various Dimorphic Fungi
- Culture Confirmation Methods
- Histoplasma capsulatum
- Coccidioides immitis
- Sporothrix schenckii
- Blastomyces dermatitidis
- Paracoccidioides brasiliensis
- Match each of the names of the animals with the names of the dimorphic fungi listed that most commonly may be related to transmission of disease to hu...
- The colonies shown in the upper image were obtained on blood agar from a sputum specimen after 10 days of incubation at 30°C. The lower image is ...
- The growth of the colonies shown in the upper image was obtained on blood agar from a sputum specimen after incubation at 30°C. The lower image i...
- The colonies shown in the upper image, obtained from a biopsy of an ulcerating skin lesion of the arm, are growing on agar slants of Sabouraud's dextr...
- The upper image shows a subculture of a mold colony suspected to be a dimorphic fungus inoculated onto the surface of blood agar and incubated for 3 d...
- Although care should be taken when working with all fungal cultures in the laboratory, personnel are particularly prone to develop laboratory-acquired...
- Which dimorphic fungus may produce black, yeast-like colonies after prolonged incubation at 37°C?
- Match each of the names of the dimorphic fungal species with its mold form.
- The images below are lactophenol blue mounts of the yeast forms of various dimorphic fungi. Which of the images is consistent with an identification o...
- Differentiating Dimorphic Molds from Saprophytic Counterparts
- Differentiating Dimorphic Fungi from Saprophytic Molds
- Inhibiting the Growth of Saprophytic Molds
- This image illustrates a lactophenol blue mount of a mold recovered after 7 days of incubation in brain heart infusion broth. The large macroconidia s...
- Which of the following ingredients is added to culture media to enhance the recovery of the dimorphic fungi by preventing the overgrowth of more rapid...
- References
Additional Information
Level of Instruction: Intermediate
Intended Audience: Medical laboratory scientists, medical
laboratory technicians, microbiologists, laboratory supervisors, and laboratory managers. This
course is also appropriate for MLS and MLT students and pathology residents.
Author Information:
Elmer W. Koneman, MD, received his graduate degree from the University of Colorado School of Medicine in Denver, Colorado, and until his recent passing, was a Professor Emeritus at their Department of Pathology. He was the author of numerous journal articles and books, including Color Atlas and Textbook of Diagnostic Microbiology, 7th Edition, 2017.
Margaret Reinhart, MS, MLS(ASCP), has been the MLS Program Director and Senior lecturer in Biological Sciences at the University of the Sciences in Philadelphia, PA, where she taught hematology, clinical immunology, parasitology, and other related courses. She is also an adjunct instructor in Hematology at Pennsylvania Hospital, Philadelphia, PA. She holds a Master's Degree in Biology and in Health Care Administration.
The authors have no
conflict of interest to disclose.
Reviewer Information:
Laurie Bjerklie, MA, MLS(ASCP)CM, is
a Lead Education Developer. She earned a B.S. in Medical Laboratory Science
from the University of North Dakota and an M.A. in Curriculum and Instruction
from Saint Xavier University. She has over 15 years of experience in higher
education and has held program director and faculty positions in both MLT and
MLS programs.
Dr. Julie Ann West is certified by the American Society for Clinical Pathology (ASCP) as a Medical Laboratory Scientist (MLS) and as a Specialist in Microbiology (SM). In addition, Dr. West has earned a PhD in Public Health - Infectious Disease Epidemiology - and is Certified in Public Health (CPH) by the National Board of Public Health Examiners. Dr. West is experienced as a Technical Specialist, Safety Officer, Educator, and Lead in the Veterans Administration Healthcare System, and has prior experience as an Administrative Laboratory Director.
