Mycology: Hyaline and Dematiaceous Fungi (Online CE Course)

Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1.5 hour(s) Approved through 8/31/2024

Florida Board of Clinical Laboratory Personnel Credit Hours - General (Microbiology/Mycology/Parasitology): 1.5 hour(s) Approved through 8/31/2024

Objectives

• Identify hyaline mold isolates by observing the appearance of the colony on agar plates and through microscopic appearance.
• Discuss various sources of fungal infections and the diseases that are associated with hyaline and dematiaceous molds.
• Define these terms that apply to fungi and are used to identify specific species: sporulation, conidia, conidiophore, hyphae, phialide.
• Identify dematiaceous molds by observing characteristic structures and their rate of growth.
• Identify the common environmental sources (natural habitats) of certain hyaline and dematiaceous molds
• Discuss some of the types of diseases associated with opportunistic infections by some of the hyaline and dematiaceous molds.

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Course Outline

• Introduction
• Introduction
• Clinical Classifications
• Pathogens vs. Opportunists
• Morphological Classifications
• Definitions and terms
• Structures
• Diseases and clinical conditions
• Hyaline Molds
• Hyaline molds
• The Mucorales (former Zygomycetes)
• Microscopic remarks for the Mucorales (Zygomycetes):
• The Dermatophytes
• Diagnosis and Identification of Dermatophytes
• The Opportunistic Mycoses Caused by Other Hyaline Molds
• Diagnosis and Identification of Opportunistic Mycoses Caused by Other Hyaline Molds
• Other notes - Hyaline Molds that Appear Similar to the Mold Phases of Obligate Pathogens
• Match each hyaline mold from the drop-down list to its corresponding colony and microscopic description. The mold colonies are illustrated in the imag...
• Match each hyaline mold from the drop-down list to its corresponding microscopic and colony description. The microscopic appearance of the molds are i...
• Match each hyaline mold from the drop-down list to its corresponding colony and microscopic description. The mold colonies are illustrated in the imag...
• Match each of the names of the fungi listed in the left column with its most likely associated disease listed in the right column.
• Match each of the names of the hyaline molds listed with the environmental conditions or natural objects with which it is most likely associated.
• Match the names of each of the species of hyaline molds listed with the appropriate category.
• Match the names of each of the species of hyaline molds listed with the appropriate category.
• Match the names of each of the species of hyaline molds listed with the appropriate taxonomic category.
• Match the names of each of the fungi listed below into the appropriate category indicating the classification of infection with which it is most commo...
• The microscopic features shown in this image is of a fungus commonly associated with:
• Based on the structures observed in this image, the most probable species of the fungus recovered from an induced sputum specimen is:
• Illustrated in this image is a "green lawn" colony of Gliocladium species. What is the other hyaline mold that produces this type of colony?
• The fungus illustrated in this image was recovered from an induced sputum specimen from a 74-year-old man with chronic obstructive pulmonary disease (...
• The dermatophytic fungus shown in this image was recovered from a ringworm lesion of the skin on the back of the hand of an 8-year-old boy. What is th...
• An Aspergillus species was recovered from a sputum specimen of a patient with X-ray evidence of fungal pneumonia. Microscopic examination did not perm...
• What is the identification of the hyaline mold shown in this image?
• The differentiation between Aspergillus species and Scedosporium species may be difficult when only hyphal elements are observed in stained tissue sec...
• A dermatophyte that produces thin-walled, two or three-celled macroconidia, and no microconidia, most likely belongs to which genus?
• The bare fruiting heads shown here, characteristic of Aspergillus niger, demonstrate a spherical vesicle with phialides positioned around the entire c...
• A dull white fungus, turning mouse gray on maturity, was recovered from material aspirated from a bone cyst in the upper femur. Based on the microscop...
• The "birds on a fence" arrangement of uniform-sized, tear-shaped microconidia is characteristic of:
• The image demonstrates the fruiting heads of Trichoderma species. Note the single, long, tapered phialides (arrow), extending laterally from either si...
• Illustrated here is a single conidium of Microsporum canis. Note that the hilar cell appears fractured (shorter red arrow), where it was released from...
• The hyaline saprobic fungus that has microscopic features similar to the mold form of Histoplasma capsulatum is:
• The hyaline saprobic fungus that has microscopic features similar to the mold form of Coccidioides immitis is:
• The most helpful feature in differentiating the Zygomycetes/Mucorales from the other hyaline molds in the clinical mycology laboratory is:
• Dematiaceous Molds
• Dematiaceous Molds with Clinical Significance
• Dematiaceous Molds with Clinical Significance, continued
• Diagnosis and Identification of Slow-Growing Molds
• Diagnosis and Identification of Fast-Growing Molds
• Other Diagnostic Methods for Dematiaceous Molds
• Match each of the names of the fungal species listed with the corresponding identifying structures illustrated in the images:
• Match the names of each of the fungal species listed in the drop-down box with the corresponding identifying structures shown in the images:
• A presumptive identification of the four genera of slower growing pathogenic dematiaceous molds can be made by observing specific types of conidiation...
• Match the name of each dematiaceous fungus listed in the drop-down box with its most likely disease.
• Match the name of each fungal species listed with its most likely corresponding morphologic features.
• Match the name of each fungal species listed in the drop-down box with its most likely corresponding morphologic feature.
• Match the names of each of the fungi listed with its appropriate category.
• Match the names of each of the fungi listed with its appropriate category depending upon whether the multi-celled macroconidia are divided by both lon...
• Match the names of each of the fungi listed with its appropriate category indicating the degree of pathogenicity.
• The fungal species most likely associated with the granulomatous infection seen in this image, illustrating segmented, dark brown-staining grains with...
• The infrequently encountered mold that is represented by this image begins as a gray-white colony that blackens with maturity as the hyphae become dar...
• The multi-celled conidia of this dematiaceous mold are divided into cells by what are called distosepta (pseudosepta), indicating that the individual ...
• The oval or curved multi-celled, dark-staining macroconidia divided by transverse septa only is characteristic of:
• What is the type of sporulation of the dematiaceous mold that is shown in this image called?
• Of the following dematiaceous fungi, the black, suede-like colony illustrated here, reaching no larger than the size of a dime after 7 days incubation...
• The dematiaceous colony illustrated here grew to a diameter of 3 - 4 cm in 5 days. The dematiaceous fungus that can be ruled out is:
• The dematiaceous fungus that may produce both acrotheca and rhinocladiella types of sporulation is:
• The black yeast colony illustrated in this photograph may represent any of the following dematiaceous molds except:
• The chain of conidia illustrated in this photomicrograph, with the deep-staining truncated bases, are called annelloconidia, and are most characterist...
• In this image are observed several background dematiaceous hyphae within which is seen a long, flask-shaped, tapered phialide (arrow) that has a flat ...
• The disease with which the dematiaceous fungus illustrated in this image is most likely associated is:
• The dematiaceous conidium shown in this image was obtained from a tiny portion of dark colony that grew to maturity in six days. Spores incubated in a...
• The chief microscopic feature possessed by Ulocladium species by which it can be differentiated from the close look-alike Stemphylium species is:
• References
• References

Additional Information

Level of instruction: Intermediate