Course Outline
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- Significance: Reservoir, Pathogenesis, and Epidemiology of Medically Important Aerobic Actinomycetes
- Introduction: Background, Classification, and Safety
- Reservoir
- Pathogenesis
- Cutaneous and Subcutaneous Forms
- Pulmonary Forms
- Disseminated Forms
- Epidemiology
- The reservoir of actinomycetes is:
- Which aerobic actinomycetes is most commonly encountered among medical cases in the United States?
- Laboratories should use which Biosafety Level when processing aerobic actinomycetes?
- Laboratory Preparation of Specimens
- General characteristics and Specimen Preparation
- All of the following are true of specimen preparation for isolation of the aerobic actinomycetes, except:
- Laboratory Identification: Microscopy - Staining Techniques and Reactions
- Microscopy: Staining Techniques
- Microscopy: Staining Reactions
- Microscopy: Sulfur granules
- A modified-Kinyoun acid-fast staining technique uses:
- When viewing a direct Gram stain of suspected aerobic actinomycetes, all of the following may be seen, except:
- Laboratory Identification - Morphology and Testing
- Media for use in Propagating Aerobic Actinomycetes
- Primary Media
- Selective Media
- Primary isolation medium for sterile body site specimens include:
- After initial growth is achieved, subcultures of suspected aerobic actinomycetes to media should include which different incubation temperatures to de...
- Traditional Methods of Phenotypic Characterization
- Phenotypic Characterization
- Media and Methods Used for Identification
- More about Lysozyme...
- More about Decomposition of Substrates...
- All of the following tests may be used to aid in the phenotypic characterization of the aerobic actinomycetes, except:
- Decomposition of substrates among the aerobic actinomycetes refers to:
- Genotypic Identification Methods
- Chromatography
- Molecular Methods
- PCR: NAATs and RFLP
- Other Molecular Tests for Aerobic Actinomycetes
- In the identification of aerobic actinomycetes, HPLC methods are:
- Which of the following is a true statement?
- Serological Assays and MALDI-TOF
- Serological Assays
- MALDI-TOF
- Regarding the identification of aerobic actinomycetes - select the true statement from the list below:
- Regarding MALDI-TOF - select the false statement from the statements listed below:
- Description of Genera and Species of Medically Important Aerobic Actinomycetes
- Genera and Species
- Nocardia
- Streptomyces
- Rhodococcus
- Gordonia
- Actinomadura
- Tsukamurella
- Williamsia
- Other Similar Organisms...
- Choose the statement which is true about Nocardia:
- Regarding Rhodococcus: Which statement is false?
- Susceptibility Testing and Treatment of Human Aerobic Actinomycetes Infections
- Susceptibility Testing: Methods & Limitations
- Susceptibility: Typical Profiles
- Treatment
- A Note about Prevention
- Regarding susceptibility testing methods of the aerobic actinomycetes, which statement is false?
- For Nocardia, Streptomyces, and Actinomadura infections, the usual drug(s) of choice is(are):
- References
Additional Information
Level of Instruction: Intermediate/Advanced
Intended Audience: Medical technologists, and instructors experienced in bacteriology, graduate students in microbiology, clinical pathologists, and infectious disease physicians.
Course Description: This course describes the significance of medically important aerobic actinomycetes. Microscopy, morphology, and identification methods are discussed. Susceptibility testing and treatment of infections are also included in this module. This module is intended for medical microbiologists having basic bacteriology experience.
Author Information: Dr. Julie Ann West is certified by the American Society for Clinical Pathology (ASCP) as a Medical Laboratory Scientist (MLS) and as a Specialist in Microbiology (SM). In addition, Dr. West has earned a PhD in Public Health - Infectious Disease Epidemiology - and is Certified in Public Health (CPH) by the National Board of Public Health Examiners. Dr. West is experienced as a Technical Specialist, Safety Officer, Educator, and Lead in the Veterans Administration
Reviewer Information: Nicole Darius is certified by the American Society for Clinical Pathology (ASCP)CM as a Medical Laboratory Scientist. In addition, Nicole is experienced as a Microbiology Supervisor, Safety and Biosafety Officer, Antimicrobial stewardship and Infection Control member, Lean certified, and Quality Assurance specialist in the Veterans Administration Healthcare System. She also has previous experience as a generalist medical technologist in Chemistry, Hematology, Urinalysis, and Blood Bank.
