Course Outline
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- Introduction
- Basic Staining Mechanisms
- What are Dyes?
- Factors that Affect Dye Binding
- Acids, Bases, pH, and Buffers
- Basophilic and Acidophilic Staining
- Oxidation and Reduction
- Tissue staining is affected by all of the following factors except:
- A solution with a pH of 6.0 is categorized as which of the following?
- Connective Tissue Classification and Functions
- What is Connective Tissue?
- Classifying Connective Tissue
- Functions of Connective Tissue
- Where are reticular fibers (reticulin) found?
- True or False: Cartilage is composed of collagen, elastin, and reticulin fibers, as well as adipose tissue.
- Special Staining Methods Used to Demonstrate Connective Tissue
- Using the Microwave for Special Staining Procedures
- Microwaves in the Histopathology Laboratory
- Tips for Using the Microwave for Special Staining Procedures
- Safety Precautions for Microwave Usage
- True or False: Containers used in a microwave oven should be tightly sealed to prevent liquids from bubbling.
- What should users do to prevent high-dose exposure to microwaves?
- References
Additional Information
Level of Instruction: Intermediate
Intended Audience: Clinical laboratory histotechnologists, histotechnicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and medical laboratory students, pathology residents, and practicing pathologists.
Author Information: Tanisha N. Neely, HT(ASCP), is a freelance writer with a work history in histology, microbiology, and pharmaceutical research. She attended Indiana University, where she earned a bachelor's degree in arts and humanities from the School of General Studies and a Certificate in Histotechnology from the School of Medicine. She is a member of the National Society for Histotechnology and the Indiana Society for Histotechnology.
Reviewer Information:
Diana Harrington, BS, HT(ASCP), is a histotechnologist at The Dermatology Center of Indiana. Since graduating from Indiana University, she has worked in various hospital laboratories as a medical technologist and histotechnologist. Her experience led her to teach IU students as a Clinical Education Supervisor and create the Histotechnology Program as Program Director for Keiser University in Florida.
Carla J. Shoffeitt, MSM, HT(ASCP), is the Manager of the Pathology Department of Emory St. Joseph’s Hospital of Emory Healthcare in Atlanta, Georgia. She holds a Master of Science Degree in Healthcare Management as well as a Bachelor of Science degree in Biology from Troy University, Troy, Alabama. She is certified as a Histotechnician and has over 25 years of experience in the field of Histology.
Course Description: This is the second in a series of courses that will explore special stains used in the clinical histology laboratory. It will discuss basic connective tissue staining mechanisms and classify connective tissue elements. The material presented details the chemistry, diagnostic application, and staining protocols for special stains used to demonstrate connective tissue in samples.
