Course Outline
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- Introduction
- Basic Staining Mechanisms
- What Makes a Dye?
- Factors That Affect Dye Binding
- Acids, Bases, pH, and Buffers
- Basophilic and Acidophilic Staining
- Oxidation and Reduction
- Which of the following dye-binding factors is primarily responsible for reorganizing certain molecular structures in tissue samples so that they are m...
- A staining solution with a pH of 5.5 would be considered:
- Carbohydrate Classification and Tissue Detection
- Carbohydrates
- Classifying Carbohydrates
- Monosaccharides
- Polysaccharides
- Mucopolysaccharides
- Diagnostic Value of Demonstrating Mucopolysaccharides
- What is the primary polysaccharide demonstrated in tissue samples via histology staining techniques?
- What are mucopolysaccharides most often referred to as?
- Special Staining Methods Used to Demonstrate Carbohydrates in Tissue Samples
- Periodic Acid-Schiff: Diagnostic Applications
- Periodic Acid-Schiff: Chemistry
- Periodic Acid-Schiff: Staining Protocol
- Periodic Acid-Schiff with Diastase: Diagnostic Applications
- Periodic Acid-Schiff with Diastase: Chemistry
- Periodic Acid-Schiff with Diastase: Staining Protocol
- Periodic Acid-Schiff: Counterstaining with Hematoxylin
- Periodic Acid-Schiff: Counterstaining with Fast Green
- Place the following periodic acid-Schiff (PAS) staining procedure steps in order.
- Mucicarmine: Diagnostic Applications
- Mucicarmine: Chemistry
- Mucicarmine: Staining Protocol
- Place the following mucicarmine staining procedure steps in order.
- How should mucicarmine stock solution be stored if it will be stored for an extended period of time?
- Alcian Blue: Diagnostic Applications
- Alcian Blue: Chemistry
- Alcian Blue pH 1.0 Staining Protocol
- Alcian Blue pH 2.5 Staining Protocol
- Colloidal Iron: Diagnostic Applications
- Colloidal Iron: Chemistry
- Colloidal Iron: Staining Protocol
- Appropriate Controls for Carbohydrate Staining Methods
- Which special staining technique is most widely used to demonstrate glycogen in tissue samples?
- What is the carminic acid that gives mucicarmine its rose-red color extracted from?
- Using the Microwave for Special Staining Procedures
- References
Additional Information
Level of Instruction: Intermediate
Intended Audience: Clinical laboratory histotechnologists and technicians, and other medical laboratory personnel who have an interest in this subject matter. This course is also appropriate for histology and medical laboratory science students, pathology residents, and practicing pathologists.
Author Information: Tanisha N. Neely, HT(ASCP) is a global histology technical liaison for Covance Central Laboratory Services. Prior to this position, she worked in hospital laboratories in histology and microbiology departments. Ms. Neely attended Indiana University where she earned an associate's degree in General Studies and a Certificate in Histotechnology. She is a member of the National Society for Histotechnology and the Indiana Society for Histotechnology.
Reviewer Information: Laurie Bjerklie, MA, MLS(ASCP)CM, is
currently an Education Developer for MediaLab and LabCE. She earned a B.S. in
Medical Laboratory Science from the University of North Dakota and an M.A. in
Curriculum and Instruction from Saint Xavier University. She has over 15 years
of experience in higher education and has held program director and faculty
positions in both MLT and MLS programs.
Course Description: This course is the first in a series of courses that will explore special stains used in the clinical histology laboratory.
