Course Outline
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- Molecular Diagnostics
- Overview
- Overview, cont.
- Targets
- Basis of Molecular Testing
- Nucleotides
- Gene Expression
- Types of RNA
- Human Genome
- Which of the following types of RNA is responsible for amino acid transport?
- Which of these nucleotide bases is present in DNA but is replaced by Uracil in RNA?
- Terms
- Unique Terminology
- Terms and Definitions
- Match the following terms with the most appropriate response:
- The three base nucleotide sequence that provides the information necessary to identify an amino acid is termed a(n):
- Specimen Collection & Handling
- Overview
- Specimen Collection and Handling
- Pre-examination Variables
- Transport
- Resources
- All of the following are examples of pre-examination variables that affect molecular methodologies, except:
- When collecting blood samples, one anticoagulant to avoid, especially when performing PCR is:
- Methodology Classifications
- Classification
- Classification, cont.
- Targets of interest may include any of the following, except:
- Classification-specific Procedures
- Overview
- Direct Nucleic Acid Testing
- Amplified Nucleic Acid Testing
- Which of the following is not an example of an amplification method?
- Procedure Principles
- Direct Nucleic Acid Testing Principle
- Direct Nucleic Acid Tests
- Amplified Nucleic Acid Testing Principle
- Amplified Nucleic Acid Tests
- Amplification
- Thermal Cycling
- Amplification Issues
- Hybridization
- Factors Affecting Hybridization
- Detection
- Match the following detection techniques with the most appropriate description:
- Which of the following steps is not included in a direct nucleic acid test?
- Match the following tests to their appropriate principle:
- Clinical Applications
- Clinical Applications Overview
- Why Choose a Molecular Method?
- Selection
- When Nucleic Acids Become Altered
- Infectious Diseases
- Pharmacogenomics
- Genotypic Assays
- Advantages of Molecular Testing
- Disadvantages of Molecular Testing
- What is the name of the substitution nucleic acid alteration that causes a coding for a different amino acid?
- Which of the following is considered an advantage of molecular testing?
- References
Additional Information
Level of instruction: Intermediate
Intended Audience: Clinical laboratory technologists and technicians, and other health care personnel who have an interest in this subject matter. This course is also appropriate for clinical laboratory science students and pathology residents.
Author Credentials: Lori Woeste, EdD, MS is an Assistant Professor in the Clinical Laboratory Science Program at Illinois State University in Normal, Illinois. She holds a Master's degree in Health and a Doctorate in curriculum and instruction. She has taught introductory and advanced courses in clinical chemistry and clinical biochemistry.
Reviewer Credentials: David Moffa, PhD, has over 30 years of experience in the health care industry as an executive manager, clinical laboratory director, and medical laboratory scientist. He is currently a technical consultant for Kentmere Healthcare, Wilmington, DE, and until his retirement, was the Regional Director for LabCorp, Inc. He holds a PhD in medical biochemistry from the School of Medicine, West Virginia University.
Reviewer Credentials: Dr. Julie Ann West is certified by the American Society for Clinical Pathology (ASCP) as a Medical Laboratory Scientist (
MLS) and as a Specialist in Microbiology (SM). In addition, Dr. West has earned a PhD in Public Health - Infectious Disease Epidemiology - and is Certified in Public Health (CPH) by the National Board of Public Health Examiners. Dr. West is experienced as a Technical Specialist, Safety Officer, Educator, and Lead in the Veterans Administration Healthcare System, and has prior experience as an Administrative Laboratory Director.
Course Description: This course provides an introduction to the basic principles of molecular diagnostics and the associated terminology. Several direct and amplified nucleic acid test methods are presented and clinical applications are discussed.
