Different Processing Techniques in the Histology and Mohs Laboratory: A Comparative Approach to Tissue Handling and Workflow (Online CE Course)

Author: Camille McKay, M.Ed., BSHCA, HTL, CLT
Reviewer: Brandon Possum, HT(ASCP)

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
Approved through 5/31/2028
Approved through 5/31/2028

Objectives

  • Differentiate between histology and Mohs roles in patient care.
  • Compare and contrast tissue-handling workflows, including fixation and processing, embedding, sectioning, and staining.
  • Explain the purpose behind key technical steps in each laboratory.
  • Recognize and correct the misconception that Mohs is a diagnostic tool.
  • Apply appropriate workflow practices in histology and Mohs settings.

Course Outline

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Additional Information

Level of Instruction: Basic  
Intended Audience: Histotechnologists, histotechnicians, pathologists' assistants, laboratory supervisors, and laboratory managers. This course is also appropriate for HTL and HT students, cytologists, and pathology residents.
Author Information: Camille Pellone-McKay, M.Ed., BSHCA, HTL, CLT, has been the regional pathology manager at Memorial Sloan Kettering (MSKCC) for 10 years, overseeing the Mohs laboratories and the breast pathology laboratory. Camille has worked within the pathology/Mohs field for more than 40 years. Camille is currently the President of the American Mohs Society of Histotechnology (ASMH 2025–2026). Camille, along with colleagues Dr. Nehal and the MSK Mohs team, has authored a textbook titled The Laboratory Manual for Mohs Micrographic Surgery: Frozen Tissue Processing. Camille has a consulting business where she prepares laboratories to be compliant. Camille is also a Fulbright Specialist with the United States Government and an Adjunct Professor at SUNY Broome, teaching histology. 
The author has no conflict of interest to disclose.
Reviewer Information: Brandon Possum, HT(ASCP), is a histotechnologist with over a decade of specialized experience in Mohs histology at Memorial Sloan Kettering Cancer Center. He is board-certified by the American Society for Clinical Pathology (ASCP) and holds a New York State Histotechnician (HT) license. Brandon began his career with a strong foundation in traditional histology through his formal college education before advancing into the specialized field of Mohs frozen sectioning. In addition to his clinical work, Brandon is actively engaged in professional education. He regularly participates in speaking engagements and educational sessions focused on Mohs histology.
Course Description: This course introduces the fundamental differences between routine histology and Mohs micrographic surgery. It will explore how each laboratory functions, compare workflows, and understand the distinct roles of diagnosis versus treatment in patient care. 

Figure 2. Mohs micrographic surgery


Figure 13. Whole mount Mohs tissue


Figure 11. Relaxing incision made parallel to the wound edge to help the tissue lie flat