Course Outline
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- Introduction
- Predictive Testing is the Basis of Personalized Treatment
- Predictive versus Prognostic
- Response Rates to Common Therapies
- True or False: A prognostic factor is information concerning the patient used to help the clinician select the appropriate individuals for treatment w...
- True or False: Researchers have determined that only patients with tumors that demonstrate more significant than 70% estrogen receptor (ER) positive c...
- Types of Breast Cancer
- Incidence and Risk Factors
- Breast Cancer Classification
- Tumor Staging
- Use of Immunohistochemistry for Breast Tumor Classification
- True or False: Adenocarcinoma is a very rare type of human breast cancer.
- True or False: Obesity, alcohol, and tobacco use are believed to have an impact on the risk of developing breast cancer.
- Regulation and Compliance with Predictive Markers
- Validation, Quality Control, Regulations...Why?
- Regulation
- Validation Purpose
- Compliance Documentation
- Mandatory Proficiency Testing
- True or False: Laboratories accredited by the College of American Pathologists (CAP) that perform HER2 testing are not required to participate in prof...
- True or False: Test method verification or validation is required for all new test systems used in the laboratory before they are used in patient test...
- Theoretical Basis of Immunohistochemical Testing
- Basics of Immunohistochemistry
- Variables Affecting IHC
- Types of Antibodies
- Staining Patterns
- Which factors may impact staining by IHC techniques?
- True or False: IHC alone is used to determine a primary diagnosis.
- Hormone Receptor Testing
- Human Epidermal Growth Factor Receptor 2
- HER2 Testing by In Situ Hybridization
- Introduction
- Specimen Handling and Fixation for Molecular and In Situ Methods
- Introduction to HER2 Methods Using In Situ Hybridization
- Introduction to Fluorescent In Situ Hybridization Methods
- Fluorescence
- Details of Fluorescent In Situ Hybridization
- Chromogenic In Situ Hybridization Method for HER2
- HER2 Test Method: Advantages and Disadvantages
- CISH and FISH Scoring Criteria
- Using chromogenic in situ hybridization (CISH), a single copy of HER2 amplification appears under the light microscope as which of the following?
- What is the most commonly used, rapid, and cost-effective method to assess HER2 status in clinical histology?
- References
Additional Information
Level of Instruction: Intermediate
Intended Audience: This course is intended for histology bench technicians and technologists, supervisors, and administrators.
Author Information: Joelle Weaver MAOM, HTL(ASCP), QIHC, is a certified histotechnologist with a broad range of practical histology experience in clinical histopathology. She has received qualification in immunohistochemistry (IHC) from the American Society for Clinical Pathology and Green Belt certification from Purdue University, West Lafayette, Indiana. Joelle's bachelor's degree was received from Bowling Green State University in Bowling Green, Ohio, and her Master's Degree was awarded from Bluffton University in Bluffton, Ohio. She graduated from a NAACLS-accredited histotechnology program and has held positions as an adjunct professor, education coordinator, and program director at Columbus State Community College in Columbus, Ohio (2002-2011). Joelle is a histotechnologist and technical consultant in Louisville, Kentucky, specializing in IHC.
Reviewer Information: Carla J. Shoffeitt, MSM, HT(ASCP), is the Manager of the Pathology Department of Emory St. Joseph’s Hospital of Emory Healthcare in Atlanta, Georgia. She holds a Master of Science in Healthcare Management and a Bachelor of Science in Biology from Troy University, Troy, Alabama. She is certified as a Histotechnician and has 25 years of experience in the field of Histology.
Course Description: Toxicity, side effects, and unpredictable effectiveness have been the hallmarks of anti-cancer therapies used in the past to treat breast and other primary cancers. Targeted therapies have been developed to reduce patient risk and improve patient outcomes. This course will discuss how predictive markers/testing select patients most likely to respond positively to a specific breast cancer treatment, including current testing methodologies and compliance standards.
