Course Outline
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- Introduction
- Methods Used for Antibody Detection and Identification
- Indirect Antiglobulin Test (IAT)
- Tube Method
- Gel Method
- Solid Phase Method
- Significance of Reactions at Different Phases of Testing
- Products Used to Facilitate Antibody Identification
- Effect of Enzymes and Dithiothreitol (DTT)
- Clinically significant antibodies react best at which phase of testing?
- Antibody Detection and Identification
- Initial Steps for Identifying an Antibody
- Initial Observations of Antibody Panel
- Rule-Out Procedures
- Rule-Out Procedures, continued
- Ruling Out Example
- Ruling Out Example, continued
- Ruling Out Example, continued
- Case Study: Immune Alloantibody
- Case Study: Immune Alloantibody, continued
- Case Study: Immune Alloantibody, continuedUsing the information from the case study on the previous two pages, what antibody matches the pattern of re...
- Rule Out Procedures: Selecting Additional Rule-Out Cells
- Example of Selecting Additional Rule-Out Cells
- Based on initial serologic testing, a patient is suspected to have an anti-K, but anti-E cannot be ruled out. In this case, which of the following is ...
- Picking Selected Panel Cells Conservatively
- Rule-Out Procedure Summary Guidelines
- These antibody panel results were obtained on a patient sample. Which of the following antibodies could account for all of the reactions?Rh-HrKellDuff...
- Differentiate Between Naturally Occurring and Immune Antibodies
- Naturally Occurring Antibodies vs. Immune Antibodies
- Example of Naturally Occurring Antibody
- Example of Clinically Significant Immune Antibody
- Alloantibodies vs. Autoantibodies
- Naturally occurring antibodies may be due to exposure to which of the following?
- Advanced Antibody Identification
- Antibodies to High Prevalence and Low Prevalence Antigens
- Conclusion
- References
Additional Information
Level of Instruction: Intermediate
Intended Audience: Medical laboratory scientists, technicians, and pathologists. This course is also appropriate for medical laboratory science students and pathology residents.
Author Information: Margaret Alba, DOM, CLS(NCA), BB(ASCP) is a Lecturer in Clinical Immunohematology at the University of New Mexico in Albuquerque, New Mexico, and a Medical Technologist at Tricore-University Hospital, also located in Albuquerque. She holds a Master's in Oriental Medicine from the International Institute of Chinese Medicine and a Bachelor of Science degree in Medical Technology from the University of Texas at El Paso.
Reviewer Information: Chelsey Geurkink, MS, MLS(ASCP)CMSBBCM, is an instructor for the Specialist in Blood Banking and Medical Laboratory Science program within the College of Health Sciences at RUSH University. She completed her Bachelor of Science at the University of Wisconsin-Madison in Biology and Bachelor of Science in Medical Laboratory Science at RUSH University. She completed her graduate degree in Clinical Laboratory Management at RUSH University. Chelsey received the Teaching Assistant of the Year award in the Medical Laboratory Science Department at RUSH University in 2015 and was recognized for her accomplishment in education and training in the field of transfusion medicine by the American Association of Blood Banks in 2019. She is currently the Manager of RUSH University Medical Center's Blood Bank.
Course Description: This course will take you through methods that can be used in the process of identifying an antibody in transfusion medicine. Many examples are presented that take you step-by-step through these procedures and provide you with appropriate panel results.
