Immunoassay Techniques Used in Drug Test Screening: Enzyme Multiplied Immunoassay Technique (EMIT)

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The page below is a sample from the LabCE course Drug Testing Methods in the Clinical Toxicology Laboratory. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Immunoassay Techniques Used in Drug Test Screening: Enzyme Multiplied Immunoassay Technique (EMIT)

The enzyme multiplied immunoassay technique (EMIT) is one of the more common drug screening platforms in use today. It was developed by the Syva Company in 1970. In this enzyme immunoassay (EIA), nicotinamide adenine dinucleotide (NAD) is reduced to NADH when it reacts with the enzyme G6PD. A change in absorbance is measured at 340 nm by a spectrometer. See the figure below.