Tuberculin Skin Test (TST): Procedure

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The page below is a sample from the LabCE course Latent Mycobacterium tuberculosis Infection and Laboratory Test Methods. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Tuberculin Skin Test (TST): Procedure

Tuberculin Skin Test (TST)
Procedure (Note -This synopsis is intended to provide a basic understanding of the TST procedure; to provide greater detail is beyond the scope of this course.)
1. The standard TST is performed using the Mantoux technique, where 0.1 mL of 5 tuberculin units of tuberculin purified protein derivative (PPD) is injected intradermally (into the top layers of skin) on the forearm (also known as the volar surface) using a 27-gauge needle and a tuberculin syringe.
2. Trained providers read the skin test after 48–72 hours.
3. The presence or absence and the diameter of induration (hard bump) are measured in millimeters.
7. CDC. (2016). Administering the TST. Slide #23 from Targeted Tuberculosis (TB) Testing and Treatment of Latent TB Infection. https://www.cdc.gov/tb/publications/slidesets/ltbi/default.htm
8. CDC. (2016). Reading the TST. Slide #24 from Targeted Tuberculosis (TB) Testing and Treatment of Latent TB Infection. https://www.cdc.gov/tb/publications/slidesets/ltbi/default.htm

Administering the TST. (7)
Reading the TST. (8)