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Yersinia pestis
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Preliminary Identification of the Primary Select Agents of Bioterrorism (retired 9/7/2022)
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Yersinia pestis
Culture Characteristics:
Growth at 22-25
o
C and at 35
o
C
Organism prefers 25
o
C, so the colonies will grow faster at this temperature
Growth occurs on most routine media, including sheep blood agar (SBA), chocolate agar (CHOC), MacConkey (MAC) agar, and routine blood culture media
Non-lactose fermenter on MAC agar
Growth is slower than other
Enterobacteriaceae
Colony Morphology on SBA at 35
o
C:
At 24 hours, colonies are pinpoint and translucent with a gray-white color
Colonies take on a yellow tint as they age, after 48-72 hours, referred to as a "hammered copper" appearance
An irregular or "fried egg" appearance, shown in the lower image, can also be seen at 48-72 hours
Characteristic Features:
Growth at 22-25
o
C is a hallmark feature of this organism
Y. pestis on SBA 48h image courtesy of CDC
Y. pestis "fried egg" on SBA at 72h image courtesy of CDC
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