Cytochemical Tests Used in Differentiating Myeloid Cells from Lymphocytic Cells

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Cytochemical Tests Used in Differentiating Myeloid Cells from Lymphocytic Cells

Table 3 lists some of the cytochemical stains used in differentiating lineages (there are additional stains not listed here).
Table 3. Cytochemical Tests Used in Differentiating Myeloid Cells from Lymphocytic Cells
StainSite of ActionCells StainedCommentsImage
Myeloperoxidase Primary Granules
Auer Rods
Myeloblasts; Granulocytes;
Monocytes slightly positive
Separates AML+ from ALL-
(3)
Sudan Black BPhospholipids Myeloblasts; Granulocytes;
Monocytes slightly positive
Separates AML+ from ALL-

(4)
Naphthol AS-D Chloroacetate/(CAE) - specific esteraseCytoplasm Neutrophilic granulocytes;
Mast cells
Separates AML+ from ALL-

(5)
Periodic acid-Schiff Stain (PAS)Glycogen Granular pattern with negative background in lymphoblasts
Positive in erythroleukemia, abnormal erythrocyte precursors, and ALL
(6)
3. Giri, Dhurba. "Myeloperoxidase (MPO) Stain: Purpose, Principle, Procedure and Interpretation." LaboratoryTests.org, 6 Nov 2018, http://laboratorytests.org/myeloperoxidase-mpo-stain/
4. Giri, Dhurba. "Sudan Black B Stain: Purpose, Principle, Procedure and Interpretation." LaboratoryTests.org, 18 Nov 2018, http://laboratorytests.org/sudan-black-b-stain/
5-6. Naphthol AS-D chloroacetate/(CAE) - specific esterase and periodic acid-Schiff stain images courtesy of Dr. Jui-Han Huang, MD.