Table 3 lists some of the cytochemical stains used in differentiating lineages (there are additional stains not listed here).
Table 3. Cytochemical Tests Used in Differentiating Myeloid Cells from Lymphocytic CellsMyeloperoxidase | Primary Granules Auer Rods | Myeloblasts; Granulocytes; Monocytes slightly positive | Separates AML+ from ALL- | (3) |
Sudan Black B | Phospholipids | Myeloblasts; Granulocytes; Monocytes slightly positive | Separates AML+ from ALL-
| (4) |
Naphthol AS-D Chloroacetate/(CAE) - specific esterase | Cytoplasm | Neutrophilic granulocytes; Mast cells | Separates AML+ from ALL-
| (5) |
Periodic acid-Schiff Stain (PAS) | Glycogen | Granular pattern with negative background in lymphoblasts
| Positive in erythroleukemia, abnormal erythrocyte precursors, and ALL | (6) |
3. Giri, Dhurba. "Myeloperoxidase (MPO) Stain: Purpose, Principle, Procedure and Interpretation." LaboratoryTests.org, 6 Nov 2018, http://laboratorytests.org/myeloperoxidase-mpo-stain/ 4. Giri, Dhurba. "Sudan Black B Stain: Purpose, Principle, Procedure and Interpretation." LaboratoryTests.org, 18 Nov 2018, http://laboratorytests.org/sudan-black-b-stain/ 5-6. Naphthol AS-D chloroacetate/(CAE) - specific esterase and periodic acid-Schiff stain images courtesy of Dr. Jui-Han Huang, MD.