Cervista HPV DNA ((Third Wave Technologies, Hologic Inc., Madison, WI)

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The page below is a sample from the LabCE course Human Papillomavirus (HPV) and Molecular Testing for Cervical Cancer (retired 2/17/2021). Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Human Papillomavirus (HPV) and Molecular Testing for Cervical Cancer (retired 2/17/2021) (online CE course)
Cervista HPV DNA ((Third Wave Technologies, Hologic Inc., Madison, WI)

Two assays are available:
1. Cervista HPV HR Screening Test
14 HR-HPV Types: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, or 68
2. Cervista HPV 16/18
Identifies and differentiates HR Types 16 and 18, referred to as HPV-16/18 genotyping test
In the Cervista HPV DNA assays, two hybridizations and an enxyme cleavage occur in a primary reaction. The secondary reaction is detection with signal amplification using fluorescence resonance energy transfer (FRET). Prior to hybridization, the HPV DNA (if pressent) is isolated, denatured, and added to microplate wells.